Fast MultiSite Mutagenesis System

Features • Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination. • Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis. • Efficient: >90% mutagenesis efficiency. Notes We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles. Storage at -20°C for two years Kit Content Component FM201-01 (10 rxns) 2×TransStart® FastPfu PCR SuperMix 1 ml DMT Enzyme (10 units/μl) 30 μl 2×Assembly Mix 50 μl DMT Chemically Competent Cell 10×50 μl ddH2O 1 ml Papers citing this product: Literature Journal IF Author Date Link Regulation of Antimicrobial Peptides in Aedes aegypti Aag2 Cells FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 5.218 Zhang R, et al. Tsinghua-Peking Center for Life Sc 2017 Feb The original link The cellular distribution of Na+/H+ exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer Oncotarget 5.008 Du G, et al. Department of Biochemistry and Molecu 2016 May The original link Improving the catalytic efficiency of Bacillus pumilus CotA-laccase by site-directedmutagenesis applied microbiology and biotechnology 3.376 Chen Y, et al. The Key Laboratory of Industrial Bi 2016 Nob The original link Fascin phosphorylation sites combine to regulate esophageal squamous cancer cell behavior Amino Acids 3.173 Fa?Min Zeng,et al. 2017May The original link Identification and characterization of two types of amino acid-regulated acetyltransferases in actinobacteria bioscience reports 2.9059 Yu-Xing Lu,et al. 2017Jul The original link